Förster resonance energy transfer microscopy and spectroscopy for localizing protein–protein interactions in living cells

Y Sun, C Rombola, V Jyothikumar… - Cytometry Part …, 2013 - Wiley Online Library
The fundamental theory of Förster resonance energy transfer (FRET) was established in the
1940s. Its great power was only realized in the past 20 years after different techniques were …

Augmentation of CFTR maturation by S-nitrosoglutathione reductase

…, M Forbes, S Rehman, V Jyothikumar… - … of Physiology-Lung …, 2016 - journals.physiology.org
S-nitrosoglutathione (GSNO) reductase regulates novel endogenous S-nitrosothiol signaling
pathways, and mice deficient in GSNO reductase are protected from airways hyperreactivity…

Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy

V Jyothikumar, Y Sun… - Journal of biomedical …, 2013 - spiedigitallibrary.org
A method to investigate the metabolic activity of intracellular tryptophan (TRP) and
coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster …

Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

V Jyothikumar, EJ Tilley, R Wali… - Applied and …, 2008 - Am Soc Microbiol
Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not
only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before …

Hypoxia-induced changes in protein S-nitrosylation in female mouse brainstem

…, K Brown-Steinke, S Gunter, V Jyothikumar… - American Journal of …, 2015 - atsjournals.org
Exposure to hypoxia elicits an increase in minute ventilation that diminishes during continued
exposure (roll-off). Brainstem N-methyl-D-aspartate receptors (NMDARs) and neuronal …

Cardiolipin synthase is required for Streptomyces coelicolor morphogenesis

V Jyothikumar, K Klanbut, J Tiong… - Molecular …, 2012 - Wiley Online Library
The fluid mosaic model has recently been amended to account for the existence of
membrane domains enriched in certain phospholipids. In rod‐shaped bacteria, the anionic …

Tryptophan as an alternative biomarker for cellular energy metabolism

V Jyothikumar, Y Sun… - Natural Biomarkers for …, 2014 - api.taylorfrancis.com
7.1 Introduction e importance of autofluorescence for studying cells and tissues lies mainly
in its potential for diagnostic applications (Acuna et al. 2009; Berberan-Santos 2008; Monici …

[PDF][PDF] Label Free Fluorescence Lifetime FRET Imaging

V Jyothikumar, Y Sun, A Periasamy - Biophysical Journal, 2013 - cell.com
Multiphoton microscopy with endogenous contrasts in biological tissues have primarily
focused on detecting signals from the reduced nicotinamide adenine dinucleatide (NADH), its …

Airway epithelial hemoglobin β regulates ciliary endothelial NOS

N Marozkina, G Piedimonte, L Cottrell, V Jyothikumar… - 2013 - Eur Respiratory Soc
Introduction: Patients with primary ciliary dyskinesia (PCD) and asthmatic obesity can have
airway inflammation but low FENO. Endothelial hemoglobin (Hb) α and metHb reductase (…

Förster Resonance Energy Transfer Microscopy for Monitoring Molecular Dynamics in Living Cells

V Jyothikumar, Y Sun, A Periasamy - … of Fluorescence Imaging, 2019 - taylorfrancis.com
Förster resonance energy transfer (FRET) is an effective and high-resolution method to
monitor protein-protein interactions in live or fixed specimens. FRET imaging can be …