Turn-on fluorescence sensing of nucleoside polyphosphates using a xanthene-based Zn (II) complex chemosensor
A Ojida, I Takashima, T Kohira, H Nonaka… - Journal of the …, 2008 - ACS Publications
Fluorescence sensing with small molecular chemosensors is a versatile technique for
elucidation of function of various biological substances. We now report a new fluorescent …
elucidation of function of various biological substances. We now report a new fluorescent …
Rational design of FRET-based ratiometric chemosensors for in vitro and in cell fluorescence analyses of nucleoside polyphosphates
Y Kurishita, T Kohira, A Ojida… - Journal of the American …, 2010 - ACS Publications
Ratiometric fluorescence sensing is a useful technique for the precise and quantitative analysis
of biological events occurring under complex conditions, such as those inside cells. We …
of biological events occurring under complex conditions, such as those inside cells. We …
Organelle-localizable fluorescent chemosensors for site-specific multicolor imaging of nucleoside polyphosphate dynamics in living cells
Y Kurishita, T Kohira, A Ojida… - Journal of the American …, 2012 - ACS Publications
ATP and its derivatives (nucleoside polyphosphates (NPPs)) are implicated in many
biological events, so their rapid and convenient detection is important. In particular, live cell …
biological events, so their rapid and convenient detection is important. In particular, live cell …
Cooperation between artificial receptors and supramolecular hydrogels for sensing and discriminating phosphate derivatives
S Yamaguchi, I Yoshimura, T Kohira… - Journal of the …, 2005 - ACS Publications
This study has successfully demonstrated that the cooperative action of artificial receptors
with semi-wet supramolecular hydrogels may produce a unique and efficient molecular …
with semi-wet supramolecular hydrogels may produce a unique and efficient molecular …
A comprehensive quantification method for eicosanoids and related compounds by using liquid chromatography/mass spectrometry with high speed continuous …
M Yamada, Y Kita, T Kohira, K Yoshida… - … of Chromatography B, 2015 - Elsevier
Fatty acids and related metabolites, comprising several hundreds of molecular species, are
an important target in disease metabolomics, as they are involved in various mammalian …
an important target in disease metabolomics, as they are involved in various mammalian …
Phosphoprotein-selective recognition and staining in SDS-PAGE by bis-Zn (II)-dipycolylamine-appended anthracene
A Ojida, T Kohira, I Hamachi - Chemistry letters, 2004 - academic.oup.com
A novel fluorescence detection system using a chemosensor for phosphoprotein in gel
electrophoresis analysis has been developed. The system employed bis-Zn(II)-dipycolylamine (…
electrophoresis analysis has been developed. The system employed bis-Zn(II)-dipycolylamine (…
Characterization of supported liquid extraction as a sample pretreatment method for eicosanoids and related metabolites in biological fluids
T Kohira, Y Kita, SM Tokuoka, M Shiba… - … of Chromatography B, 2019 - Elsevier
Sample pretreatment is an important process in liquid chromatography-mass spectrometry-based
quantitative lipidomics. Reversed-phase solid phase extraction (RP-SPE) has been …
quantitative lipidomics. Reversed-phase solid phase extraction (RP-SPE) has been …
Recognition and fluorescence sensing of specific amino acid residue on protein surface using designed molecules
A Ojida, Y Miyahara, T Kohira… - … : Original Research on …, 2004 - Wiley Online Library
Many biological processes are mediated by surface recognition between proteins. Small
molecules that recognize and bind a specific region of a protein surface may be promising …
molecules that recognize and bind a specific region of a protein surface may be promising …
Novel swine model of transfusion‐related acute lung injury
H Okazaki, O Ishikawa, T Iijima, T Kohira… - …, 2014 - Wiley Online Library
Background Transfusion‐related acute lung injury ( TRALI ) is a life‐threatening complication
of blood transfusion. Antibodies against human leukocyte antigens in donors' plasma are …
of blood transfusion. Antibodies against human leukocyte antigens in donors' plasma are …
Real-time off/on-mode fluorescence assay for enzyme reactions involving nucleoside polyphosphates by use of a xanthene ZnII-Dpa chemosensor
… Takahiro Kohira, Ippei Takashima, Hiroshi Nonaka, Akio Ojida, and Itaru Hamachià Department
of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto …
of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto …