Broad-range PCR amplification of part of the 16S rRNA gene followed by single-strand
sequencing was applied to samples of 18 resected heart valves from patients with infective …

A large number of different protocols for the efficient isolation of highly purified DNA from
eukaryotic and prokaryotic cells is extant.(1-4) These procedures usually include treatment with …

Whipple's disease is a systemic illness caused by a specific agent. Despite recognition of
bacteria in lesions, efforts to isolate the causative agent remained futile. A novel strategy was …

Mycoplasma pneumoniae is a common cause of respiratory tract infections with community-acquired
pneumonia as the major disease-related burden. Compared with other pathogens, …

Toxigenic Corynebacterium diphtheriae is an important and potentially fatal threat to patients
and public health. During the current dramatic influx of refugees into Europe, our objective …

The clinical and epidemiologic management of the SARS-CoV-2 pandemic is critically
dependent on molecular assays with short turn-around time. We validated the novel Xpert Xpress …

PCR-based detection of Mucorales species could improve diagnosis of suspected invasive
fungal infection, leading to a better patient outcome. This study describes two independent …

Epidemiologically linked clusters are confirmed by typing strains with molecular typing such
as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-…

Lymphogranuloma venereum (LGV), the invasive infection of the sexually transmissible infection
(STI) Chlamydia trachomatis , is caused by strains from the LGV biovar, most commonly …

Background The increasing incidence of candidemia and emergence of drug-resistant
Candida species are major concerns worldwide. Long-term surveillance studies are needed. …