[HTML][HTML] Involvement of distinct G-proteins, Gpa2 and Ras, in glucose-and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae

…, M Crauwels, A Teunissen, D Nauwelaers… - The EMBO …, 1998 - embopress.org
Adenylate cyclase activity in Saccharomyces cerevisiae is dependent on Ras proteins. Both
addition of glucose to glucose-deprived (derepressed) cells and intracellular acidification …

Nutrient-induced signal transduction through the protein kinase A pathway and its role in the control of metabolism, stress resistance, and growth in yeast

…, L Kraakman, K Lemaire, P Ma, D Nauwelaers… - Enzyme and microbial …, 2000 - Elsevier
… -Gpa2 system only responds to D-glucose and not to L-glucose, fructose, 2-deoxyglucose,
or other analogs tested. β-D-glucose was twice as effective as α-D-glucose. The apparent K a …

Kelch-repeat proteins interacting with the Gα protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast

…, W Louwet, R Geladé, D Nauwelaers… - Proceedings of the …, 2006 - National Acad Sciences
… cAMP (C) and trehalase (D) activity were measured after addition of 100 mM glucose. … 1D)
and lowered trehalose levels in a tpk1 w mutant containing WT BCY1, indicating that the wimp …

Antiviral and lung protective activity of a novel respiratory syncytial virus fusion inhibitor in a mouse model

…, T Van de Casteele, D Nauwelaers… - European …, 2011 - Eur Respiratory Soc
Respiratory syncytial virus (RSV) causes bronchiolitis in young children and common colds
in adults. There is no licensed vaccine, and prophylactic treatment with palivizumab is very …

Pharmacokinetics-pharmacodynamics of a respiratory syncytial virus fusion inhibitor in the cotton rat model

…, D Roymans, L de Zwart, D Nauwelaers… - Antimicrobial agents …, 2010 - Am Soc Microbiol
Human respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections
in infants, young children, elderly persons, and severely immunocompromised patients. …

Relative prognostic value of self-reported adherence and plasma NNRTI/PI concentrations to predict virological rebound in patients initially responding to HAART

…, A Cozzi-Lepri, A Ammassari, MP Trotta, D Nauwelaers… - 2004 - journals.sagepub.com
We studied the predictive value of self-reported adherence and plasma drug concentrations
on virological rebound to HAART. Among 238 participants in the AdICoNA study who had …

Development and performance of conventional HIV-1 phenotyping (Antivirogram®) and genotype-based calculated phenotyping assay (virco® TYPE HIV-1) on …

T Pattery, Y Verlinden, H De Wolf, D Nauwelaers… - Intervirology, 2012 - karger.com
… The initial HXB2D clone amplicon was 872 bp long and was digested with BalI, removing
the 5 52-bp tail. This digested amplicon was ligated in the pGEMT3 Δ PRT( ApaI, BalI) vector …

Deletion of SFI1, a novel suppressor of partial Ras–cAMP pathway deficiency in the yeast Saccharomyces cerevisiae, causes G2 arrest

P Ma, J Winderickx, D Nauwelaers, F Dumortier… - Yeast, 1999 - Wiley Online Library
When glucose is added to Saccharomyces cerevisiae cells grown into stationary phase or on
non‐fermentable carbon sources a rapid loss of heat stress resistance occurs. Mutants that …

Development, evaluation, and integration of a quantitative reverse-transcription polymerase chain reaction diagnostic test for Ebola virus on a molecular diagnostics …

…, R Thijs, PB Jahrling, D Nauwelaers… - The Journal of …, 2016 - academic.oup.com
Background. The 2013–2016 Ebola epidemic in West Africa resulted in accelerated
development of rapid diagnostic tests for emergency outbreak preparedness. We describe the …

Development of a real-time multiplex RSV detection assay for difficult respiratory samples, using ultrasone waves and MNAzyme technology

D Nauwelaers, L Vijgen, C Atkinson, A Todd… - Journal of clinical …, 2009 - Elsevier
BACKGROUND: Elderly infected with Human Respiratory Syncytial Virus (RSV) often bear
low viral loads that stay below the detection limits of commercial assays. A more sensitive …