… Labeled probe (3 × 10 6 cpm) was hybridized to 5 μg of total RNA for 16 hours at 56C.
The protected RNA duplexes were purified by phenol-chloroform extraction and ethanol …

… In our patients with ABCA3 deficiency, we observed that SP-C and SP-B expression levels
varied considerably across patients. Indeed, less severe patients had decreased amounts of …

… 60 C for annealing temperature for 50 s, and 72 C … C initial denaturing for 3 min, followed
by 40 cycles denaturing temperature for 40 s, 62 C for annealing temperature for 30 s, and 72 C …

The authors have recently shown that the transcription factor nuclear factor-κB (NF-κB) is a
central mediator in the NaCl-mediated interleukin (IL)-;8 production by human airway …

… C as initial denaturing temperature for 5 min. The cycling conditions were 94 C for 30 s, 55
C for 30 s and 72 C … followed by a final extension at 72 C for 7 min. Primer sequences were 5…

… Variants in the TGFB1 gene at position +869T/C demonstrated a significant association with
lung function decline. A less pronounced rate of decline in forced expiratory volume in 1 sec …

The clinical course of cystic fibrosis (CF) varies between patients bearing identical CFTR
mutations, suggesting the involvement of modifier genes. We assessed the association of lung …

… supplemented with 100 units/ml penicillin, 100 μg/ml streptomycin (Invitrogen, Cergy-Pontoise,
France), and 10% fetal calf serum (Eurobio, Les Ulis, France) and cultured at 37C in a …

… The HLA 8.1 ancestral haplotype is strongly linked to the C allele of -429T>C promoter
polymorphism of receptor of the advanced glycation endproduct (RAGE) gene. Haplotype-…

This paper describes the results of model tests carried out in ice and open water conditions
to evaluate performance of the United States Coast Guard (USCG) Heavy Polar Icebreaker (…