Differential effects of extracellular matrix proteins on human airway smooth muscle cell proliferation and phenotype

SJ Hirst, CHC Twort, TH Lee - American journal of respiratory cell …, 2000 - atsjournals.org
… After thawing overnight at 4C, it was mixed to homogeneity using cooled … at 4C and then
placed in the incubator for 1 h at 37C before equilibration with FBS-free DMEM for 1 h at 37C

Human airway smooth muscle cells express and release RANTES in response to T helper 1 cytokines: regulation by T helper 2 cytokines and corticosteroids.

…, N Berkman, C Witt, CH Twort… - … (Baltimore, Md.: 1950 …, 1997 - journals.aai.org
… , UK) at 95C for an initial 5 min, followed by 24 cycles of denaturation at 95C for 30 s,
annealing at 55C for 30 s, and extension at 72C for 60 s. Final extension was 10 min at 72C. The …

Portable liquid oxygen and exercise ability in severe respiratory disability.

RM Leach, AC Davidson, S Chinn, CH Twort… - Thorax, 1992 - thorax.bmj.com
BACKGROUND: The development of portable liquid oxygen systems, capable of delivering
high flow rate oxygen for long periods, justifies reassessment of the value of supplemental …

Hypoxic vasoconstriction in rat pulmonary and mesenteric arteries

…, TP Robertson, CH Twort… - American Journal of …, 1994 - journals.physiology.org
Hypoxic vasoconstriction was investigated in isolated pulmonary and mesenteric arteries of
the rat. Experiments were performed on large (approximately 2 mm pulmonary, …

β2‐Adrenoceptor agonists inhibit release of eosinophil‐activating cytokines from human airway smooth muscle cells

MP Hallsworth, CHC Twort, TH Lee… - British journal of …, 2001 - Wiley Online Library
… Cells were seeded at 5×10 5 viable cells in 25 cm 2 culture flasks and maintained in a
humidified atmosphere at 37C in 5% CO 2 , with the medium replaced every 72 h. Using …

Cyclic guanosine monophosphate-enhanced sequestration of Ca2+ by sarcoplasmic reticulum in vascular smooth muscle.

CH Twort, C Van Breemen - Circulation research, 1988 - Am Heart Assoc
The purpose of this study was to investigate the effects of the intracellular messenger cyclic
GMP (cGMP) on sequestration of cytosolic calcium (Ca2+) into the intracellular Ca2+ store (…

PDGF isoform-induced proliferation and receptor expression in human cultured airway smooth muscle cells

SJ Hirst, PJ Barnes, CH Twort - American Journal of …, 1996 - journals.physiology.org
… at 37C although this was not the case at 4C. This may also explain the apparent discrepancy
observed in our experiments between the ability of the heterodimer to stimulate ASM …

Quantifying proliferation of cultured human and rabbit airway smooth muscle cells in response to serum and platelet-derived growth factor

SJ Hirst, PJ Barnes, CHC Twort - American journal of respiratory cell …, 1992 - atsjournals.org
… for 3.5 h at 37C. The resulting blue formazan product (seen as blue crystals within the cell
cytosol under the light microscope) was solubilized overnight (16 h) at 37C by the addition of …

Effect of changing dietary sodium on the airway response to histamine.

PG Burney, JE Neild, CH Twort, S Chinn, TD Jones… - Thorax, 1989 - thorax.bmj.com
The airway response to histamine has been shown to be related to the 24 hour urinary
excretion of sodium. To assess whether this relation is likely to represent a direct causal …

Cultured human airway smooth muscle cells stimulated by interleukin-1 β enhance eosinophil survival

MP Hallsworth, CPC Soh, CHC Twort… - American journal of …, 1998 - atsjournals.org
… After 30 min incubation at 37C, the tissue was transferred into a similar enzyme mixture
containing 3 U/… atmosphere at 37C in 5% CO 2 /95% air. Fresh medium was replaced every 72 h. …