Abstract
Considering the concept of M1/M2 programming macrophages can obtain pro-inflammatory M1 or anti-inflammatory M2 phenotype and change the phenotype in the disease formation. One of significant regulators of macrophages activity is surfactant protein D (SP-D).
Objective: Assessment of SP-D level and oligomeric forms in BALF in patients with asthma (A), gastroesophageal reflux disease (GERD) with pulmonary manifestations and A+GERD combination vs. healthy volunteers (HV).
Methods: SP-D level in BALF was assessed by ELISA (BioVendor, κaT. No 194-0591). Oligomeric SP-D forms were analysed by Western blot analysis (tris-acetate gels, Invitrogen, NuPAGE, # EA03752BOX).
Results: SP-D level in BALF in A patients was 1.80 times increased and in GERD - 2.66 times decreased vs patients with combination of A and GERD (748.32±69.25 ng/ml, 155.83±18.13 ng/ml vs 414.72±50.22 ng/ml, p<0.05); and 1.4 times increased and 3.42 times decreased vs. HV (748.32±69.25 ng/ml, 155.83±18.13 ng/ml vs 533.20±21.12, p<0.05). Oligomeric SP-D forms in GERD and A+GERD showed predominance of monomeric forms vs A and HV with monomeric and multimeric SP-D oligomers.
Conclusion: In asthma, GERD with pulmonary manifestations and their combination quantitative and qualitative composition of SP-D in BALF vary against healthy volunteers and each other. In asthma patients SP-D level was increased vs HV. GERD was characterized by maximum decreased SP-D level. Oligomeric forms of SP-D in asthma were similar to HV. In GERD and asthma+GERD oligomeric SP-D composition was significantly changed vs HV but with no significant differences between the groups.
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