Abstract
Our previous studies demonstrated the role of cyclin D1 in pulmonary arterial smooth muscle cells (PASMCs) proliferation. In this study, we observed the role of neuron-derived orphan receptor 1 (NOR1) in the regulation of cyclin D1 and PASMCs proliferation.
Fetal bovine serum (FBS) induced the proliferation of human PASMCs in a dose-dependent way (figure 1A, *P<0.05 as compared with control). 10% FBS induced a maxima NOR1 expression at 2h (figure 1B and 1C, *P<0.01). Transfection of an over-expressed plasmid induced NOR1 up-regulation (figure 1D and 1E, *P<0.01) and cells proliferation (figure 1F, *P<0.05).
NOR1 over-expression significantly induced cyclin D1 up-regulation (figure 2A and 2B, *P<0.05). But, the antisense oligodeoxynucleotides (AS-ODNs) against human NOR1 gene significantly inhibited cyclin D1 expression and cells proliferation (figure 2C-2F, *P<0.05 as compared with FBS group). The negative control ODNs affect either cyclin D1 expression or cells proliferation (figure 2D and 2F, *P>0.05 as compared with FBS group).
So, we concluded that NOR1 mediated human PASMCs proliferation at least partly via cyclin D1.
- © 2014 ERS