Abstract
Background: Relevant Russian rules and regulations for TB institutions don't cover sanitary bacteriological surveillance of Mtb contamination of nosocomial objects that can serve as sources of nosocomial tuberculosis infection.
Objectives: To evaluate effectiveness of implementation of sanitary bacteriological surveillance of Mtb presence on surface of objects into the system of infectious control.
Materials and Methods: In 2012-2013 the technology of bacteriological control of Mtb presence on surfaces of nosocomial objects developed by the authors was implemented into the system of infectious control of URIPP. Sampling was made by special brush; 2 ml of Dey-Engley medium was used as a washout liquid. 1 ml of sample was used for determining presence (by IS6110) and quantity of DNA Mbt; the rest was inoculated on LJ media.
Results: In 2012 the 96,4% of studied surfaces were contaminated with DNA Mtb. In 32,1% of samples the quantity of DNA was significant (≥100-300 Mtb cells per sample), 10,9% were culture positive. The received data was used for reconsideration of anti-epidemic measures. Particularly, the disinfectant was replaced. Half a year after the replacement the monitoring of presence of DNA Mtb on the surfaces was repeated. The results showed that the percentage of positive DNA Mtb samples reduced by 42,2%; the percentage of surfaces significantly contaminated by DNA Mbt reduced by 25,1%, the percentage of culture positive samples reduced by 3,9%.
Conclusions: The implementation of sanitary bacteriological surveillance of Mtb presence on the surfaces of objects of TB hospital allowed to evaluate potential epidemiological hazard during 1-2 days and take effective anti-epidemic measures.
- Copyright ©ERS 2015