Abstract
Mast cell numbers or activity levels could help identify asthma subtypes and be relevant for treatment effectiveness http://ow.ly/Xmanp
The heterogeneity of asthma phenotypes represents a challenge for adequate assessment and treatment of the disease [1]. The molecular analysis of highly characterised cohorts of asthma patients should help to identify biomarkers of asthma subtypes, which might lead to more efficient and personalised therapies for asthma [1, 2]. So far, asthma has been mainly divided into two subtypes: T-helper cell type 2 (Th2)-high asthma, which comprises patients with airway and systemic eosinophilia who are responsive to glucocorticoids; and Th2-low asthma, which comprises patients without airway or systemic eosinophilia who are not responsive to glucocorticoids [3]. Besides eosinophilia, patients with Th2-high asthma have other signs of airway type 2 inflammation, including increased numbers of airway mast cells [4].
Many phenotypic characteristics of mast cells can be modulated by genetic and environmental factors, including changes in the microenvironment associated with the progression of a disease [5]. Despite such phenotypic plasticity, mast cells have been generally subcategorised into two groups based on the protease content of their cytoplasmic granules: MCT, which express high levels of tryptase but little or no chymase; and MCTC, which express both tryptase and chymase [6, 7] as well as cathepsin G and carboxypeptidase A3 (CPA3) [8, 9]. While MCTC are predominantly found in connective tissues, such as the skin, MCT are found at mucosal sites and represent the majority (∼90%) of lung mast cells. MCT are found in the bronchial/bronchiolar lamina propria and alveoli [10], and low numbers of MCTC can be found beneath the epithelium in the lamina propria and submucosa, in close proximity to submucosal glands, and around the airway smooth muscle layers of major bronchi [11].
Gene expression analysis of airway epithelial brushings has identified two mast cell-specific protease genes (the tryptase TPSAB1 and CPA3) among the top 10 most differentially expressed genes in asthmatic versus control subjects [12]. A follow-up study further revealed that among asthmatics, patients with Th2-high asthma have significantly higher intraepithelial mast cell numbers in the airways than patients in the Th2-low group, and that such intraepithelial mast cells display a unique phenotype with expression of tryptase and CPA3 but not chymase [4]. Importantly, expression of tryptase and CPA3 in epithelial brushings was associated with responsiveness to glucocorticoids [4], which reveals that such mast cell-specific proteases represent potential biomarkers of asthma subtypes. However, the molecular phenotyping method used in that study to identify mast cell protease genes required bronchoscopic samples, and therefore has limited applicability in the clinic [4].
One approach that could be more broadly applicable is the analysis of gene expression in induced sputum [13]. In this issue of the European Respiratory Journal, Wang et al. [14] demonstrate that gene expression for tryptase and CPA3 can be detected in induced sputum cells from asthmatics. The authors defined three groups of patients: one without significant levels of mast cell protease gene expression, a second in which only tryptase could be detected (MCT) and a third with significant expression of both tryptase and CPA3 genes (which they named MCT/CPA3). Chymase expression was low in all three groups, suggesting that luminal mast cells also have the same unusual protease phenotype (tryptase and CPA3 high and chymase low [13]) that is found in intraepithelial mast cells in Th2-high asthma [4]. These results for chymase are also in agreement with a previous report, which also identified tryptase and CPA3 gene expression in induced sputum cells from asthmatics [13]. In the present report, Wang et al. [14] confirm and extend these results by showing that the “MCT/CPA3” subtype has elevated exhaled nitric oxide fraction, sputum eosinophils, bronchial sensitivity and reactivity compared with the “MCT” subtype. In addition, the authors report that the “MCT/CPA3” subtype had better clinical response to corticosteroid treatment.
A limitation of the current study by Wang et al. [14] is the absence of quantification and characterisation of mast cells in sputum by immunohistochemistry. Although present at very low levels, mast cells have been detected in induced sputum of asthmatics in two previous studies [15, 16]. Both studies also noted that basophils were present in sputum from asthmatics (and at higher numbers than mast cells). Circulating basophils express tryptase, albeit at much lower levels (<1%) than that found in tissue-resident mast cells [15, 17, 18]. In addition, recent studies in mice showed that some levels of CPA3 promoter activity can be detected in basophils [19, 20]; whether this is also the case in human basophils is unknown. Together, these results raise the possibility that the tryptase and CPA3 gene expression that is detectable in induced sputum might originate from basophils rather than mast cells.
The finding that higher gene expression for both tryptase and CPA3 in induced sputum is associated with eosinophilic asthma and responsiveness to glucocorticoids is clinically relevant for at least two reasons. The first reason is the possibility that mast cell genes could be measured as a biomarker of Th2-high asthma or of responsiveness to corticosteroids. The problem here is that measuring gene expression in induced sputum cells is relatively complex and is ill-suited as a test in a patient care environment. A more feasible biomarker strategy would be blood-based measures of mast cell proteases, but further study is necessary to determine how well blood measures of tryptase or CPA3 reflect airway measures. The second reason is the possibility that mast cells (or basophils) orchestrate type 2 immune responses in the airway, i.e. that they are upstream rather than downstream of type 2 cytokine activity. Specifically, it is possible that mast cells or basophils are important cellular sources of the type 2 cytokines that cause the airway eosinophilia in Th2-high asthma. In this scenario, the work of Wang et al. [14] provides rationale for considering mast cells and basophils as cellular targets to treat Th2-high asthma.
Footnotes
Support statement: L.L. Reber is the recipient of a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2014 656086) from the European Commission and acknowledges support from the French Institut National de la Santé et de la Recherche Médicale (INSERM). J.V. Fahy acknowledges support from National Institutes of Health (National Heart, Lung, and Blood Institute) grants HL109146 and HL107202 and the Sandler Center for Basic Asthma Research. Funding information for this article has been deposited with FundRef.
Conflict of interest: Disclosures can be found alongside the online version of this article at erj.ersjournals.com
- Received January 10, 2016.
- Accepted January 11, 2016.
- Copyright ©ERS 2016