Abstract
In follow up of our studies on proteomic changes in a validated mouse model of immunologically mediated chemical-induced asthma, using toluene-2,4-diisocyanate (TDI) as a sensitizer [1 we evaluated the temporal changes at early time points following dermal sensitization. The identification of biomarkers of sensitization could help to move diagnosis to an earlier (pre-clinical) stage. We explored the proteome of the auricular lymph nodes and serum of mice dermally sensitized to TDI.
Mice were treated once (day 1) or twice (day 1 and 8) with TDI or with the vehicle (acetone-olive oil, 2:3, control) on both ears. Auricular lymph nodes and serum were collected three days later. Two-dimensional difference gel electrophoresis was used to analyze the differential proteins (p<0.01) of TDI-sensitized mice (n=12) vs. control mice (n=12).
Proteome analyses of the auricular lymph nodes resulted in 39 and 86 differential proteins and of serum in 7 and 16 differential proteins, after 1 and 2 sensitizations, respectively. Identification (MALDI-TOF MS) of these proteins mainly showed structural (e.g. vimentin), immune related (e.g. lymphocyte specific protein-1) and oxidative stress related proteins (e.g. peroxiredoxin 6) in both the lymph nodes and the serum.
Now, a software based pathway analysis of the differential proteins is performed (Ariadne Genomics). This will give more insight in the cellular and molecular events involved in early sensitization, leading to chemical-induced asthma. Possible biomarkers among the differential proteins will be validated.
Reference:
1. Haenen S, Vanoirbeek JA, De Vooght V, Maes E, Schoofs L, Nemery B, Hoet PH, Clynen E. J Proteome Res. 2010;9(11):5868-76.
- © 2011 ERS