Abstract
Introduction
One of the hypotheses of COPD development suggests protease-antiprotease imbalance,which can cause degradation of extracellular matrix. As a consequence, the decline of lung function can be observed. Matrix metalloproteinase 9 (MMP9) is a proteolytic enzyme responsible for collagen digestion, and may contribute to COPD pathogenesis. In the MMP9 gene, a -1562 C/T polymorphism was found, and the -1562T allele is associated with increasing level of promoter activity. It may be argued that the above-mentioned allele can influence incidence of COPD.
Aims and objective
To investigate the potential role of -1562C/T polymorphism in COPD development.
Eighty-three patients diagnosed with COPD according to the GOLD criteria, and 110 healthy control individuals were enrolled in the study. All cases have Polish Caucasians ethnicity.
Methods
DNA was extracted from peripheral blood using Invisorb Spin BloodMidi Kit following the manufacturer's instructions (Invitek, Germany).
The MMP9 -1562C/T polymorphism was typed by PCR-RFLP method using 5'-GCCTGGCACATAGTAGGCCC-3' and 5'-CTTCCTAGCCAGCCGGCATC-3' primers, and cutting with restriction enzyme SphI (Zhang, B. et al. Circulation, 1999; 99: 1788-1794).
Results
The patients with COPD differed from healthy controls in frequencies of MMP9 -1562C/T alleles (p=0.02) and genotypes (p=0.03). An association of -1562T allele with COPD in comparison with healthy controls (frequencies: 0.20 vs 0.10, respectively;OR=2.07, 95%CI=1.15-3.72) was observed using Fisher exact test as a statistical method.
Conclusion
The MMP9-1562T allele seems to be associated with disease susceptibility in the Polish COPD patients.
- © 2014 ERS