Abstract
Mice are used to develop models of pulmonary allergic disease.
Objective: To evaluate the increase gradual OVA doses in a protocol of experimental asthma.
Methods: Balb/c mice male were divided in Groups: Control, OVA1%, OVA3%, OVA5%. Sensitized animals received i.p.(OVA+Sal+AlumHidrox; Days: 0,14,28,42). Inhalations sessions (OVA+Saline; 3x wk/30min) were performed between 21st and 54th day. The OVA1% group received inhalation with concentration of OVA1%. The OVA3% began with 1% for 3 weeks (wk), in the 4th wk was increased for 3%. The OVA5% began with 1% in the 1st wk, 2sd wk was increased for 2%, 3rd wk with 3%, 4th wk for 4%, 5th wk for 5% of OVA. The Control group received i.p. and Saline inhalations. In the study were evaluated IgE and IgG1 titers by PCA technique, cells in BALF and peribronchial eosinophils, and smooth muscle of the airways for image analyses.
Results: IgE and IgG1 were increased in sensitized groups, but in the groups 3% and 5% the levels were highest and shown increased of cell migrations presented increase of eosinophils, neutrophils and trickiness of the smooth muscle when compared with others two groups (p<0.01), just Group 3% shown high index of peribronchial eosinophils (p<0.01).
Conclusions: Groups OVA3% and OVA5% are more efficient for develop features of experimental asthma, and seems that Group OVA3% develop important increase of eosinophil migration in the airways.
- © 2011 ERS