Abstract
Introduction: Membrane phospholipids (PL) provide functional integrity of the cells of respiratory tract.
Aim: To study of membrane phospholipids' composition of peripheral blood lymphocytes in the dynamics of phototherapy in rats with experimental COPD.
Methods: An experimental COPD was reproduced in 60 white rats under the influence of tobacco smoke. For half of them we used the method of phototherapy of concentrated pulsed light (CPL) at wavelengths from 600 to 800 nm. Phototherapy was carried out daily for 10 days. The control group consisted of 10 healthy rats. To study the phospholipids' fractions of lymphocytes we used the high-flow horizontal chromatography.
Results: In rats with experimental COPD, compared with the control group, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were reduced by 31,8% (P<0,01) and 43% (P<0,01), the content of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) was increased two-fold (P<0,01). Free fatty acids (FFA), compared with the control, increased by 47,2% (P<0,01) with a decrease in total phospholipids (TFL) by 17% (P<0,01). After 10 sessions of phototherapy we defined increase in PC and PE by 18,8% and 28,4% compared with the group of COPD without treatment, and this was accompanied by a decrease in LPC and LPE by 35,1% and 40,9% (P<0,05 in all cases). Tendency to normalization of FFA and TFL had no statistical significance.
Conclusion: Exprimental COPD in the rats is accompanied by quantitative changes in the main fractions of phospholipids in the membranes of peripheral blood lymphocytes. Conducting a course of phototherapy by CPL contributes to positive change in membrane phospholipids of these cells.
- © 2011 ERS