Abstract
Peroxisome proliferator–activated receptor (PPAR)-γ is a ligand-activated transcription factor and regulates inflammation. Many studies have indicated PPARγ can exert protective effect during acute lung injury(ALI), however, it is unclear how the PPARγ itself was regulated in this process. In this study, we investigated the role of nuclear receptor cofactor receptor interacting protein (RIP140) which was known as an important corepressor for PPARγ and its correlation with PPARγ during ALI.In our study, we found enhanced expression of RIP140 in mice of LPS-induced acute lung injury,but decreased PPARγ expression. Besides, a similar result was found in RAW264.7 macrophage after LPS stimulation. Down-regulation of RIP140 by knockdown could restore PPARγ expression and activity in macrophage and lung, and suppress the inflammatory reaction to LPS, and alleviate lung injury. However, this beneficial effect could be partially reversed by PPARγ antagonist GW9662.These findings suggest that down-regulation of RIP140 expression could suppress the inflammatory reaction and alleviate lung injury , which may be mediated by elevating PPARγ expression and activity.
- Copyright ©ERS 2015