European Respiratory Society

Figure 2.

Wed, 2022-02-23 12:53 -- admin
Summary: 
Anecdotal evidence of CLC, EETs and galectin-10 in the airways of an autoimmune-eosinophilic asthma patient. a) Scanning electron microscopy image of hexagonal CLCs (arrows) and b) electron microscopy evidence of CLCs in formalin-fixed paraffin embedded (FFPE) sputum plugs from a 55 year-old female prednisone-dependent severe asthma patient with radiological (high-resolution CT) evidence of mucus plugging, increased anti-EPX antibodies, antinuclear antibodies (in-house measurements, described in Mukherjee et al. [52]) and 7.9 ng·mL−1 of galectin-10 (commercially available assay, Novus Biologicals) detectable in the processed cell-free fraction of sputum. Images were acquired in collaboration with Canadian Centre of Electron Microscopy, McMaster University, Hamilton, ON, Canada. c) Shows FFPE plug sections stained with haematoxylin and eosin (H&E; light microscopy) and immunostained with anti-human histone monoclonal antibody and DAPI (fluorescence microscopy). Image acquisition was in collaboration with Molecular Phenotyping and Imaging Core (St Joseph's Healthcare Hamilton, ON, Canada). Immunostaining and FFPE sputum plug block methods have been described in Mukherjee et al. [120].
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Figure
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Normal
Slug: 
F42
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Contributors: 
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